Ben jemaa, Slim (2009) Fine mapping of female fertility qtl in french dairy cattle. PhD thesis GENETIQUE/GENOMIQUE ANIMALE, AgroParistech 2009AGPT0012 p.189.

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Abstract

The aim of this thesis was to map finely female fertility (FF) QTL in the three main French dairy cattle breeds Prim’Holstein, Normande and Montbeliarde. The first step consisted in detecting QTL on 78 families in 12 genomic regions. Six FF QTL were found in Prim’Holstein and two in Normande breeds. In the second step of the study, the QTL on bovine chromosomes BTA01, BTA02, BTA03 and BTA21 were mapped on a sub-sample of 41 families and the confidence interval for BTA01 and BTA03 QTL was reduced. Using a set of 437 SNP, the QTL location interval on BTA03 was reduced to a few centimorgans allowing us to select six candidate genes, which were partially sequenced for four DNA pools belonging to individuals with extreme phenotypes in order to find some intersting polymorphisms which can be validated on the whole pedigree. Several SNPs corresponding to the a priori status of the individuals for the QTL, were found in most of the candidate genes. However, since a bovine SNP chip with 54 000 SNP became available, no further validation step was carried out.

Based on fine mapping results, overrepresentation, with regard to the whole bovine genome, of genes located in QTL confidence intervals involved in biological functions in connection with reproduction, were checked. These results were also used to identify positional candidate genes that may influence dairy cattle FF.

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