Colas, Cyril (2006) Etude par spectrométrie de masse des harpagosides, des éleuthérosides et du cycloarténol, principes actifs utilisés en phytothérapie équine. PhD thesis DCMR, EP - DCMR Laboratoire des Mécanismes Réactionnels, EP/X p.310.
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Abstract
This five-chaptered manuscript is devo!
ted to t
he development of physico-chemical methods for the equine medical treatment control of Harpagophytum and Eleutherococcus, active principles used in herbal medicine. The first chapter deals with the objects of the study and presents the botanical and pharmacological properties of the plants of interest: Harpagophytum procumbens and Eleutherococcus senticosus. The second chapter details the experimental and theoretical techniques used: sample preparation, mass spectrometry coupled with liquid or gas chromatography and molecular modeling. The third chapter is presented on the basis of publications; extraction of active principles of Harpagophytum from equine biological samples, LC/MS analysis, as well as a theoretical approach of harpagide cationization by sodium ions and a metabolic approach of harpagoside are developed. A direct application of these results on eleutherosides B and E is shown in the fourth chapter, completed by a research of the active principles in feed suppl!
ements. The fifth and last chapter is the outcome of the applications, results and perspectives from the work performed during the last three years.
| Item Type: | PhD Thesis (PhD) |
|---|---|
| Thesis Supervisor: | Bouchonnet, Stéphane |
| Date: | 19 December 2006 |
| Board of examiners: | Marie-Agnès, Popot and Bruno, Le bizec and Christine, Enjalbal and Jérôme, Lemoine |
| Ecole Doctorale: | ED 447 ECOLE DOCTORALE DE L'ECOLE POLYTECHNIQUE |
| Discipline: | DCMR |
| Collection (Fonds): | EP/X |
| Institution: | EP/X |
| Department: | EP - DCMR Laboratoire des Mécanismes Réactionnels |
| Subjects: | 6. Chemistry, Physical Chemistry and Chemical Engineering |
| Uncontrolled Keywords: | Spectrometry, Spe, Harpagophytum, Eleutherococcus, Lc, Ms, Herbal medicine, Horse, Spectrométrie, Spe, Harpagophytum, Eleutherococcus, Lc, Ms, Phytothérapie, Cheval |
Table of content
Avant-Propos i
Plan Général iii
Table des Matières v
Abréviations et Acronymes xiii
Chapitre 1 - Introduction Générale 1
I. Contrôle de la Médication 3
II. Harpagophytum 13
III. Eleutherococcus 25
IV. Objectifs de la Thèse 37
Chapitre 2 - Techniques Expérimentales et Méthodes Théoriques Utilisées 39
I. Préparation dÉchantillon 41
II. Chromatographie 57
III. Spectrométrie de Masse 75
IV. Modélisation Moléculaire 123
Chapitre 3 - Harpagophytum (sur Publications) 135
I. Présentation 137
II. Préparation dÉchantillon 141
III. Analyse par LC/MS 167
IV. Approche Théorique de la Cationisation 191
V. Approche Métabolique 213
VI. Conclusion 221
Chapitre 4 - Eleutherococcus 223
I. Présentation 225
II. Conditions Expérimentales 227
III. Résultats et Discussion 233
IV. Approche Métabolique 249
V. Conclusion 255
VI. Références 257
Chapitre 5 - Conclusion Générale 259
I. Analyse dÉchantillons de Routine 261
II. Métabolisme 263
III. Compléments Alimentaires 265
IV. Perspectives 267
Annexes 269
Annexe I AORC Guidelines for the Minimum Criteria for Identification by Chromatography and Mass Spectrometry 271
Annexe II 16th International Conference of Racing Analysts and Veterinarians 277
| ID Code: | 2923 |
|---|---|
| Deposited By: | Laurence Vidament |
| Deposited On: | 06 September 2007 |
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