Goulpeau, Jacques (2006) DNA microarrays Integration in a Microfluidic Device. PhD thesis Physique et chimie des matériaux, ESPCI p.203.
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Abstract
This thesis deals with the design and the fabrication of microfluidic devices in PDMS (Polydimethylsiloxane) for biological applications. A microfluidic environment is first fabricated and basic tools are improved: a passive method that enables drawing up liquids in PDMS micro-channels is presented and was patented. Micro-pumps, a key element for complex architecture are studied from both an experimental and a theoretical point of view. Thanks to this study, performances are improved and a predictive model based on an electronic equivalence is described (published in \textit{Journal of Applied Physics}). Besides, hydrodynamic dispersion that leads to unwilling dilution of analyte during transport is studied for the particular case of micro-channels. From this study, a new method of production of concentration gradients is both theoretically and experimentally described. From this work, an original microfluidic plate-form that integrates DNA microarrays in a precisely controlled environment in term of temperature, micro-flow and real time measurements is described. Thanks to this new set up, DNA hybridization on chip and under bulk conditions are compared and results complete recent developments on the physic of hybridization on chip. Moreover, a model that describes the coupling phenomena of reaction-diffusion-advection is experimentally extended to DNA microarrays. Finally in collaboration with IGH (Institut de Génétique Humaine) in Montpellier, two new methods that measure allele ratio for the genetic disease “Down syndrome” are tested experimentally and experiments on biological samples are under process. As a conclusion, new microfluidics devices are presented here leading to original approaches for biological applications.
| Item Type: | PhD Thesis (PhD) |
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| Thesis Supervisor: | Tabeling, Patrick |
| Date: | 03 October 2006 |
| Board of examiners: | Brochard-Wyart, Françoise and Bruus, Henrik and Potier, Marie-Claude and Tabeling, Patrick and Trouchet, Daniel and Viovy, Jean-Louis |
| Ecole Doctorale: | ED 397 PHYSIQUE ET CHIMIE DES MATERIAUX |
| Discipline: | Physique et chimie des matériaux |
| Collection (Fonds): | ESPCI |
| Institution: | ESPCI |
| Subjects: | 4. Materials Science, Mechanics and Mechanical Engineering 7. Life Sciences and Engineering |
| Uncontrolled Keywords: | DNA Chip, Microfluidic, Micropump, Taylor-Aris dispersion, Hybridization, Pdms, Lab On chip, puce à ADN, Microfluidique, Micropompe, dispersion de Taylor-Aris, Hybridation, Pdms, Laboratoire sur puce |
| ID Code: | 2226 |
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| Deposited By: | Jacques Goulpeau |
| Deposited On: | 07 June 2007 |
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